Figure 5. Identifying cyclized metabolites (M14 and M15) and detoluene-ATX (M20).
Incubations without methxoyamine were conducted as described in Fig. 4. All the samples were analyzed using UHPLC-Q Exactive MS. The metabolite elucidation conditions are described in Fig. 4. (A) Chromatograms of metabolite M14 and M15 in HLM. (B) MS/MS of M14. (C) MS/MS of M15. (D) Chromatograms of metabolite M20 in HLM. (E) MS/MS of M20. (F) Formation of detoluene-ATX.