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. 2020 Sep 21;10:15396. doi: 10.1038/s41598-020-72340-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Genomic diversity of ankG. (a) The ankG alignment defines three groups, which are highlighted by colors (blue, red and green). Single base insertions, deletions and nucleotide substitutions are indicated either by dashes or highlighted in yellow. (b) AnkG amino acid sequences of three C. burnetii isolates –each representatives for its group. The first group contains the reference strain Nine Mile II (AnkG_NM) and nineteen additional strains expressing a 338 amino acid protein (blue). The second group includes thirteen isolates and is represented by C. burnetii Soyta (AnkG_Soyta) expressing a 92 amino acid protein. AnkG_Soyta is identical in the first 83 N-terminal amino acids (red), but harbors 9 different amino acids at the C-terminus (yellow) compared to AnkG_NM. The third group contains four isolates and is represented by C. burnetii strain F3 (AnkG_F3) expressing a 51 amino acid protein. AnkG_F3 has an amino acid exchange at position 11 (isoleucine to leucine) and is otherwise identical to AnkG_NM in the first 28 N-terminal amino acids (green), but contains 23 different amino acids at the C-terminus (yellow). The asterisk (*) at the C-terminus of the amino acid sequence representing the second and third group indicates premature truncation.