Figure 3.

Characterization of AnkG_Soyta-mediated apoptosis modification. (a) Schematic representation of the AnkG_NM and AnkG_Soyta truncations used. Amino acids are shown above each of the schematic diagrams. The striped area represents altered amino acids as compared to the reference sequence AnkG_NM. (b–e) CHO-FcR cells were transiently transfected with GFP or with the GFP-tagged AnkG variants indicated. (b,d) Proteins were separated by SDS-PAGE, transferred to a PVDF membrane and probed with an antibody against GFP. Depicted is one representative immunoblot of three independently performed experiments with similar results. (c,e) CHO-FcR cells expressing either GFP or the indicated GFP-tagged AnkG variants were treated with staurosporine for 4 h. The cells were fixed, permeabilized and the nuclei stained with DAPI. The nuclear morphology of at least 100 GFP-expressing cells was scored in three independent experiments. Error bars indicate ± SD. **p < 0.01; ***p < 0.001, n.s. not significant.