Figure 4.

Characterization of AnkG_F3-mediated apoptosis induction. (a) Schematic representation of the AnkG variants used. Amino acids are shown above each of the schematic diagrams. The striped area represents altered amino acids as compared to the reference AnkG_NM sequence. (b) CHO-FcR cells were transiently transfected with GFP or with the GFP-tagged AnkG variants indicated. Proteins were separated by SDS-PAGE, transferred to a PVDF membrane and probed with an antibody against GFP. Depicted is one representative immunoblot of three independently performed experiments with similar results. (c, d) CHO-FcR cells expressing GFP or the indicated GFP-tagged AnkG variants or truncations were treated with staurosporine for 4 h. The cells were fixed, permeabilized and the nuclei were stained with DAPI. The nuclear morphology of at least 100 GFP-expressing cells was scored in three independent experiments. Error bars indicate ± SD. *p < 0.05; **p < 0.01, ***p < 0.001.