Table 4.

Primers used in this study.

Primer no	Sequence*	Site
25	5′-AAGGCGCGCCAAGTAGACGTGAGACTCCC-3′	AscI
26	5′-AAGGCGCGCCTCACCGAGGACTAGACAG-3′	AscI
54	5′-CCGGATCCATGAGTAGACGTGAGACTCC-3′	BamHI
65	5′-CCGAGACTCCCACTAGCACAAT-3′	–
66	5′-CCCTCTACTCGAAAATGGCGTA-3′	–
329	5′-CCAAGATCTCTATGAGTAGACGTGAGACTCC-3′	BglII
362	5′-CCAATTGCGGCCGCTCACCGAGGACTAGACAGA-3′	NotI
737	5′-GCGCAATACGCTCAATCACA-3′	–
738	5′-CCATGGCCCCAATTCTCTT-3′	–
760	GCGCAATACGCTCAATCACA	–
761	CCATGGCCCCAATTCTCTT	–
848	5′-CCCCTCGAGCCAGTAGACGTGAGACTCCCA-3′	XhoI
1111	5′-CGGGGTACCTCATTTTCGGCTCAATCTCCTTCT-3′	KpnI
1115	5′-CGGGGTACCTCAGGCGGTACGTAGGCCCC-3′	KpnI
1116	5′-CGGGGTACCTCAACGTAGGCCCCATTTCGCC-3′	KpnI
1117	5′-CCGCATATGCCAAGTACGCCCC-3′	NdeI
1135	5′-CGGGGTACCTCACTGGAAATCCGTCTTTGGCG-3′	KpnI
a192	5′-CGCGGATCCTCAAAACGATAAATCGAATACAGTAAA-3′	BamHI
a208	5′-TTCGAGCTCGGTACCATGGACTACAAAGACCATGACGG-3´	KpnI
a209	5′-GCATCTAGAGGTACCTCACCGAGGACTAGACAGA-3′	KpnI
a343	5′-CGCGGATCCTCATTTTCGGCTCAATCTCCTTC-3′	BamHI
a808	5′-GGCTGGCGCTTACAAAGAGATAGTC-3′	–
a817	5′-GAGTAGACGTGAGACTCCCACTAG-3′	–
a818	5′-CGATAAATCGAATACAGTAAATATCGAATTCTTTGC-3′	–
a822	5′-CCGAGGACTAGACAGACAAGAGAGAG-3′	–
a891	5′-CCGAAGCGGTGTCGACATGAGTAGACGTGAGACTCC-3′	SalI
a892	5′-CCCATGCCTCAGTCGACTCACCGAGGACTAGACAGAC-3′	SalI
*Underlining indicates the location of the restriction site