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. 2020 Sep 21;11:4752. doi: 10.1038/s41467-020-18500-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Picture: Epididymal white fat from WT (+/+) and Ghrhr^Gpr101 (+/T). Bars: quantification of epididymal white fat weight normalized to total body weight (n = 6 mice/group, p = 0.0022 for males and females). b Representative CT images segmented for fat of 27-week-old WT (+/+) and Ghrhr^Gpr101 (+/T) mice (n = 6 mice per group). Total volume is in grayscale and fat volume is in red. Plain white arrows indicate subcutaneous fat and dashed arrows epididymal fat distribution. c Percentage of fat mass in WT (+/+) and Ghrhr^Gpr101 (+/T) mice (n = 6 mice per group, p = 0.0022) determined by CT-scan analysis. d Percentage of lean mass in WT (+/+) and Ghrhr^Gpr101 (+/T) mice (n = 6 mice per group, p = 0.0022) determined by CT-scan analysis. e 27-week-old WT (+/+) and Ghrhr^Gpr101 (+/T) representative histological sections of epididymal adipose tissue stained with H&E. Scale bar: 30 µm. This experiment was repeated at least three times. f Mean adipocyte area, quantified using at least four fields per whole-slide image, from at least four animals per group (p = 0.0159). g Representative liver histological sections from 27-week-old WT (+/+) and Ghrhr^Gpr101 (+/T) mice stained with ORO (n = 4 mice per group). Scale bars: 30 µm. h Picture: extracted livers from WT (+/+) and Ghrhr^Gpr101 (+/T). Bars: quantification of liver weight normalized to body weight (n = 6 mice per group, males: p = 0.0303, females: p = 0.0130). i GTT of 11 month-old WT (+/+) and Ghrhr^Gpr101 (+/T) mice. GTT was performed after 12 h of fasting. Glucose was injected IP to starved mice and blood was collected at indicated time points (0, 30, 60, and 90 min) to measure blood glucose levels (males: n = 6 mice per group, females: n = 5 mice per group). 0 min: p = 0.5173 for males and p = 0.7302 for females. 30 min: p = 0.0260 for males and p = 0.0317 for females. 60 min: p = 0.1970 for males and p = 0.0952 for females. 90 min: p = 0.1320 for males and p = 0.4206 for females. All data are presented as Mean ± S.D. For statistical analysis of all data, a two-sided Mann–Whitney test was used. ns not significantly different; *p < 0.05; **p < 0.01; ***p < 0.001. F Female, M Male.