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Halo assay of TSP1, TSP2, and TSP3. E. coli strain ATCC 700,728 was embedded in agarose. Wells (3 mm) were cut out of the agarose and loaded with 10 µL (6 mg/mL) of TSPs. Following overnight incubation at 37 °C, each TSP produced a halo, which is indicative of glycosidase activity.
