Figure 5.

Influence of expression of heterologous genes on the downstream native gene. (A) Expression of TDH3, the transgene (hGH or G-CSF), and PIF1 as a function of integration either upstream of the TDH3 promoter or elsewhere in the genome. Each point represents the average of triplicate RNA-seq measurements of expression in log₂(TPM + 1). Adjusted p-values were calculated using DESeq2. (B) Expression of TDH3, the transgene (hGH or G-CSF), and PIF1 as a function of integration either upstream of the PIF1 promoter or elsewhere in the genome.