Figure 3. The Timeless–Tipin complex shows a preference for binding G4 DNA .

Fluorescence anisotropy was used to measure the binding affinity of Timeless–Tipin for the indicated DNA sequences.

1. ssG4: G4 flanked by single‐stranded DNA; ssHP: hairpin flanked by single‐stranded DNA; ss: single‐stranded DNA (Appendix Table S2 for sequence details).
2. Binding affinity of Timeless–Tipin for a range of G4 DNA sequences (see Appendix Table S2 for sequence details and references). Single‐stranded (ss20: 5′‐6FAM-ATAAGAGTGGTTAGAGTGTA) and double‐stranded (ds20: ss20 annealed to complementary sequence) DNA were also tested as controls.

Data information: Each data point is the mean of at least 3 independent experiments and the error bars indicate one SD.