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. 2020 Jul 12;12(9):e12050. doi: 10.15252/emmm.202012050

Figure EV4. Overexpression of INSL5 promotes cell cycle progression and suppresses cell apoptosis.

Figure EV4

  • A
    The cell cycle of vector control or INSL5 overexpressing CNE1, CNE2, and HK1 cells were analyzed by flow cytometry assay.
  • B
    Statistical analysis of cell percentage in each cell cycle phase.
  • C
    Cyclin D, cyclin E, cyclin B, and p27 expression levels were detected by Western blotting in cells with or without INSL5 overexpression.
  • D
    Western blotting for c‐myc, BCL2, and BCL‐xL in CNE1, CNE2, and HK1 with or without INSL5 overexpression.
  • E
    CNE1 stable cell line was treated with DDP or 5‐FU and stained with annexin V/propidium iodide (PI), and measured by flow cytometry. Data shown are representative of three independent experiments.
  • F
    Statistical analysis of the effects of INSL5 overexpression on cell apoptosis under DDP or 5‐FU treatment.
  • G
    Western blotting for apoptosis pathway in CNE1, CNE2, and HK1 with or without INSL5 overexpression under 5‐FU treatment.
Data information: In (F), data are presented as mean ± SEM, from three different experiments, and P‐values were determined by unpaired t‐test. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance. Exact P‐values are specified in Appendix Table S4.Source data are available online for this figure.