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. 2020 Aug 4;21(9):e50446. doi: 10.15252/embr.202050446

Figure EV1. (Related to Figs 1 and 2) Analysis of a model system for primitive and metastatic melanoma, Secret3D and Proteostat analysis.

Figure EV1

  • A
    Growth curve of primitive WM115 and metastatic WM266.4 cell line (N = 3 biological replicates) measured as number of cells/time.
  • B
    Viability profile of IGR and WM cell lines after 24‐h starvation, detected by measuring cell confluence (%) and number of dead and alive cells using Muse™ Cell Analyzer.
  • C
    Number of proteins identified in the secretome by Secret3D compared to a standard protocol.
  • D
    Number of total and glycosylated peptides identified by Secret3D.
  • E
    Confocal immunofluorescence images of Proteostat (1:2,000, red spots) and DAPI staining (blue) for WM115 and WM266.4 cells. Scale bar is 10 μm.
  • F
    Quantitation of aggregates in the extracellular space in WMs. Fluorescence gain of proteins present in the supernatant treated with Proteostat reagent. (T‐test, *P < 0.05) N = 4 biological replicates. Data are mean ± SD.