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. 2020 Aug 4;21(9):e50446. doi: 10.15252/embr.202050446

Figure 2. Proteomic analysis of the secretome from primitive and metastatic melanoma cells.

Figure 2

  • A
    MS workflow of Secret3D: Secretome De‐glycosylation Double Digestion protocol.
  • B
    Scatter plot of identified and quantified proteins in the secretome of primitive IGR39 and metastatic IGR37. Red dots represent proteins that were significant with FDR < 0.05, and blue dots represent proteins with P < 0.05.
  • C
    Scatter plot of identified and quantified proteins in the secretome of primitive WM115 and metastatic WM266.4. Red dots represent proteins that were significant with FDR < 0.05, and blue dots represent proteins with P < 0.05.
  • D
    (Left) Venn diagram of the significant proteins shared by both IGR and WM cell lines. (Right) Histograms representing the metastatic and primitive signatures H/M ratios.
  • E
    KEGG pathway enrichment analysis of the significant proteins.
  • F
    Confocal fluorescence images of Proteostat (1:2,000, red spots) and DAPI staining (blue), scale bar is 10 μm.
  • G
    Quantitation of aggregates/cell in IGRs cell lines by immunofluorescence analysis, left panel; fluorescence gain of soluble proteins treated with Proteostat reagent, right panel. (T‐test analysis, *P < 0.05, N = 3 biological replicates, data are mean ± SD).