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. 2020 Aug 4;21(9):e50446. doi: 10.15252/embr.202050446

Figure EV5. (Related to Fig 6) Effect of BACE inhibition and BACE2 KD on colony formation, cell proliferation, and chemo‐sensitivity.

Figure EV5

  • A
    MTT assay for WMs treated with DMSO or NB‐360 (25 μM). N = 4 biological replicates. T‐test analysis, *P < 0.05, ***P < 0.001. Data are mean ± SD.
  • B
    Colony formation assay and relative quantitation on the right panels, for IGR39 and IGR37 cells treated with DMSO or NB‐360. N = 3 biological replicates. T‐test analysis, *P < 0.05, **P < 0.01. Data are mean ± SD.
  • C
    MTT assay of iBACE2 KD IGR37 cells treated or not with doxycycline. N = 3 biological replicates. T‐test analysis, **P < 0.01. Data are mean ± SD.
  • D
    Viability of WM melanoma cell lines treated with DMSO, doxorubicin 10 μM, NB‐360 25 μM, and the combination of doxorubicin and NB‐360 measured as % of viable cells. N = 3 biological replicates. T‐test analysis, *P < 0.05. Data are mean ± SD.