Reagent or Resource	Source	Identifier
Antibodies
Mouse monoclonal HMB‐45	Thermo Fisher	Catalog # MA5‐13232
Mouse monoclonal anti‐YAP	Santa Cruz	sc‐101199
Rabbit polyclonal anti‐HDAC2	Abcam	Ab16032
Mouse monoclonal anti‐FAK	Santa Cruz	sc‐271195
Mouse monoclonal anti‐pFAK (Y397)	Santa Cruz	sc‐81493
Mouse monoclonal anti‐Actin	Santa Cruz	sc‐47778
Chemicals, Peptides, and Recombinant Proteins
25‐hydroxycholesterol (D6)	Avanti Polar Lipids	LM‐4110
24(R/S)‐hydroxycholesterol (D6)	Avanti Polar Lipids	LM‐4110
27‐hydroxycholesterol (D6)	Avanti Polar Lipids	LM‐4114
NB‐360	Novartis	N/A
Doxorubicin	Selleck Chemicals	#S1208
ECL	Amersham	RPN2232
DAPI	Sigma‐Aldrich	CAS Number 28718‐90‐3
MUSE	Millipore	Catalogue Number MCH100102
Proteostat	Enzo Life science	ENZ‐51023
DMSO	Euroclone	EMR385258
Critical Commercial Assays+40:64
Bradford	Applied Biosystems	Cat#4368814
LightCycler® 480 SYBR Green I Master	Roche	Product No. 04707516001
SuperScript™ VILO™ cDNA Synthesis Kit	Invitrogen	Cat. No. 11754‐050 and 11754‐250
Ni‐NTA Agarose	Qiagen	Cat No./ID: 30230
Experimental Models: Melanoma Cell Lines
IGR37	DSMZ	ID ACC 237
IGR39	DSMZ	ID ACC 239
WM115	IZSBS	ID BS‐TCL74
WM266.4	ATCC	ID CRL‐1676
A‐375	IZSBS	ID BS‐TCL88
C32	IZSBS	ID BS‐TCL150
IPC‐298	DSMZ	ID ACC 251
SK‐MEL‐5	NCI‐60	ID 0507403
MeWo	ICLC	ID HTL97019
Sk‐MEL‐28	NCI‐60	ID 0507398
HEK293T	ICLC	ID HTL04001
Software and Algorithms
GraphPad Prism software	GraphPad Software	http://www.graphpad.com
Microsoft Excel 2010	Microsoft Office	N/A
Fiji	ImageJ Software	https://imagej.net/Fiji
MaxQuant	N/A	http://www.coxdocs.org/doku.php?id=maxquant:common:download_and_installation
Perseus	N/A	http://www.coxdocs.org/doku.php?id=perseus:start
Venny	N/A	http://bioinfogp.cnb.csic.es/tools/venny/
Deposited data
.RAW files of the proteomic data were deposited together with all peptides identified and parameters used for the analysis	Peptide Atlas repository	PASS01358