PTEN overexpression protects against PE-induced cardiac hypertrophy. (A) PTEN expression levels in untreated, PE-treated (100 µM) or PE-treated + pcDNA-PTEN-transfected primary cardiomyocytes. (B) Cell surface area in untreated, PE-treated (100 µM) or PE-treated + pcDNA-PTEN-transfected primary cardiomyocytes. (C) Protein and (D) mRNA expression levels of ANP and BNP in untreated, PE-treated (100 µM) or PE-treated + pcDNA-PTEN-transfected primary cardiomyocytes. PTEN, phosphatase and tensin homolog; PE, phenylephrine; ANP, natriuretic peptide A; BNP, brain natriuretic peptide; NC, negative control. *P<0.05.