Figure 2.

NCOA6 expression in primary CTBs isolated from the first‐trimester human placental villi was increased during in vitro EVT induction. CTBs were purified from placental villi at 6‐8 weeks of gestation by Percoll gradient and subsequently cultured in 1 mg/mL liquid Matrigel precoated plates to induce differentiation to EVTs. NCOA6 expression was measured 0, 24 and 48 hours after cell plating. The EVT markers HLA‐G and ITGA5 were used as indicators of EVT induction. The results are presented as the mean ± SD based on four independent experiments. The values with different letters are significantly different (P < .05). CTBs indicates cytotrophoblasts; EVT, extravillous trophoblast; HLA‐G, human leucocyte antigen‐G