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. 2020 Aug 12;4(9):e10392. doi: 10.1002/jbm4.10392

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© 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC. on behalf of American Society for Bone and Mineral Research.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 4 — Nonmutated recombinant carboxypeptidase E (rCPE) and mutated recombinant CPE (mrCPE) regulate cellular bioenergetics in U33 cells. (A) Profile of oxygen consumption (oxidative phosphorylation) measured with MitoStress assay after 24‐hour treatment with either rCPE or mrCPE. (B) Calculated rates of basal respiration, maximal respiration, and adenosine triphosphate production measured with MitoStress assay, as above. (C) An effect of rCPE and mrCPE on fatty acids dependency of U33 cells measured with FuelFlex assay. White bars = Vehicle (Veh); black bars = rCPE; gray bars = mrCPE. Both assays were repeated three times with 20 to 30 technical replicas per group; representative results of one assay are shown.