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. 2020 Aug 12;4(9):e10392. doi: 10.1002/jbm4.10392

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© 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC. on behalf of American Society for Bone and Mineral Research.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig 5 — Human skeletal stem cells (SSCs) osteoblastic differentiation correlates with increased expression of carboxypeptidase E (CPE). (A) Alkaline phosphatase enzyme activity of human SSCs at day 1 (D1) and after 12 days of growth (D12) in media supplemented with β‐glycerol phosphate (10mM), ascorbic acid (50 μg/mL), and dexamethasone (10nM). (B) The same SSCs were grown as in (A), and RNA was isolated at D12 and analyzed with RT‐PCR for human Cpe mRNA expression. ALP = alkaline phosphatase; CT = cell outgrowth from knee cartilage; MA3 and MA4 = marrow aspirates collected from iliac crest of two different subjects; MR = marrow collected from a knee representing hematopoietic marrow; YB = marrow collected from a femoral head highly enriched in adipocytes.