Table 1.
Coverage of HCPs in the early process sample
| Protein name | Accession no. | Total protein sample | Ab A | Ab B | Ab C | pI | MW (kDa) |
|---|---|---|---|---|---|---|---|
| Guanylate kinase | A0A140NBE7 |
|
|
|
|
5.7 | 85,357 |
| Aminotransferase | A0A140N655 |
|
|
|
|
5.4 | 44,163 |
| SmpA/OmlA domain protein | A0A140NFD8 |
|
|
|
|
6.6 | 15,689 |
| DNA‐directed RNA polymerase subunit beta | A0A140NGD5 |
|
|
|
|
5.2 | 34,775 |
| Thioredoxin | A0A140NCS9 |
|
|
|
|
5.5 | 36,649 |
| Beta‐galactosidase | A0A140NDN8 |
|
|
|
|
6.5 | 37,999 |
| DNA polymerase III, delta prime subunit | A0A140NAC4 |
|
|
6.2 | 40,655 | ||
| Ubiquinone/menaquinone biosynthesis C‐methyltransferase UbiE | A0A140NBU4 |
|
|
|
|
6.2 | 45,679 |
| TonB‐dependent siderophore receptor | A0A140NFD2 |
|
|
|
|
5.1 | 29,418 |
| Periplasmic serine endoprotease DegP‐like | A0A140N6M4 |
|
|
|
|
4.8 | 34,893 |
| Isochorismatase | A0A140NCD4 |
|
6.0 | 15,935 | |||
| S‐ribosylhomocysteine lyase | A0A140NCE4 |
|
|
|
|
6.3 | 96,127 |
| UPF0250 protein YbeD | A0A140N8X2 |
|
|
|
|
5.1 | 25,535 |
| Chromosomal replication initiator protein DnaA | A0A140NI72 |
|
|
|
|
5.2 | 54,069 |
| Muramoyltetrapeptide carboxypeptidase | A0A140NCB5 |
|
|
|
|
7.7 | 17,229 |
| UPF0227 protein YcfP | A0A140N6A8 |
|
|
|
|
5.2 | 51,455 |
| Chaperone protein DnaK | A0A140NBA5 |
|
|
|
|
4.9 | 61,158 |
| Alanine–tRNA ligase | A0A140N5K8 |
|
|
|
|
9.7 | 22,087 |
| Peptidylprolyl isomerase | A0A140N8V9 |
|
|
|
|
5.9 | 22,321 |
| Threonine–tRNA ligase | A0A140N4K1 |
|
|
|
|
10.3 | 25,983 |
| Acetyl‐coenzyme A carboxylase carboxyl transferase subunit beta | A0A140N7A3 |
|
|
|
|
5.6 | 26,801 |
| Alkyl hydroperoxide reductase C | A0A140N3G7 |
|
|
|
|
9.9 | 22,244 |
| Type I secretion outer membrane protein | A0A140N9D7 |
|
|
|
|
7.8 | 33,402 |
| Alcohol dehydrogenase zinc‐binding domain protein | A0A140NDZ9 |
|
|
|
|
6.8 | 26,726 |
| ABC transporter related | A0A140N5X9 |
|
|
|
|
9.4 | 23,335 |
| DNA polymerase I | A0A140N583 |
|
|
|
|
5.3 | 51,051 |
| Uncharacterized protein | A0A140N7G4 |
|
11.0 | 6,542 | |||
| Aldo/keto reductase | A0A140NEE0 |
|
|
|
7.9 | 46,633 | |
| DNA gyrase subunit B | A0A140NH65 |
|
|
|
|
4.9 | 57,329 |
| Histone family protein DNA‐binding protein | A0A140N7E6 |
|
|
|
|
5.8 | 74,014 |
Note: List includes 30 proteins out of 1,524 identified HCPs. Diagonal shading indicates identified in total protein sample without immunocapture. Gray fill indicates coverage by Ab A, B, or C. Dark gray fill indicates that the protein was identified in the sample after immunocapture with the HCP antibody, but not considered covered as the protein was also found in similar or higher amounts in the sample after immunocapture using the control antibody (i.e., a false positive). The HCP was considered covered/identified in this table if it was covered/identified by the HCP antibody in at least one out of three experiments.