FIGURE 2.

Effect of EPO on LPS‐induced upregulation of PGE2 in placenta. Placentae were collected. PGE2 levels were assessed at 12 hours after the first LPS or vehicle solution injection on GD15.The level of PGE2 was determined by immunohistochemistry. Representative photomicrographs of placental histological specimens from mice treated with normol saline + normol saline (A as control), normal saline + EPO (B), normal saline + LPS (C), and EPO + LPS (D) are shown. PGE2 was observed in placental trophoblast cells (black arrowheads). (C) showed the upregulation of PGE2 in placenta and PGE2 expressed in placental trophoblast cells. The expression of PGE2 in placenta is almost invisible in (A) (B) and (D). Original magnification, ×100 or 400. Scale bars 100 µm for original magnification, ×100. Scale bars 25 µm for original magnification, ×400