FIGURE 7.

Effects of EPO on the expression of placental PD‐L1 after LPS injection. Placentae were collected. PD‐L1 protein levels were assessed at 12 hours after the first LPS or vehicle solution injection on GD15. The level of PGE2 was determined by immunohistochemistry. Representative photomicrographs of placental histological specimens from mice treated with normol saline + normol saline (A as control), normal saline + EPO alone (B), normal saline + LPS (C), and EPO + LPS (D) are shown. (D) showed the upregulation of PD‐L1 in placenta and PD‐L1 expressed in placental trophoblast cells, including giant trophoblasts (black arrowheads). The expression of PD‐L1 in placenta is almost invisible in (A), (B) and (D). Original magnification, ×100 or 400. Scale bars 100 µm for original magnification, ×100. Scale bars 25 µm for original magnification, ×400