-
A
Schematic illustration of barasertib treatment protocol. Mice were treated with either vehicle or barasertib (40 mg/kg; twice a day) for last 2 weeks, while they were injected intradermally with either saline or bleomycin 5 days per week for total of 4 weeks.
-
B
Representative images of Masson's trichrome‐stained lung section from mice treated with vehicle and barasertib. Images were obtained at 10× magnification. Scale bar: 200 μm.
-
C
Quantification of total lung hydroxyproline in mice treated with vehicle and barasertib. *P < 0.05, ***P < 0.0005, 1‐way ANOVA (n = 7–8 mice/group).
-
D
Quantification of Col1α1, Col3α, Col5α, Col14, Col15, αSma, Ccna2, and Fas gene transcripts in total lungs of mice treated with vehicle or barasertib. *P < 0.05, **P < 0.005 ***P < 0.0005, ****P < 0.00005, 1‐way ANOVA (n = 6 mice/group).
-
E
Quantification of αSma protein levels in lung lysates from vehicle‐ and barasertib‐treated mice. Gapdh is used as loading control. Data are presented as mean ± SEM. **P < 0.005, unpaired t‐test (n = 5 mice/group).
Data information: All data were presented as mean ± SEM.
.
