Skip to main content
. 2020 Sep 4;11:2090. doi: 10.3389/fimmu.2020.02090

FIGURE 2.

FIGURE 2

Impact of LKB1 reconstitution on dsDNA sensing in co-culture with MVNs. (A) Confocal images at 10×, 20×, and 40× magnification of MVNs formed from HUVECs and hLFBs co-cultured with H1355 tumor spheroids within collagen/fibrin hydrogel after 7 days, immunostained for F-actin (red) and EpCAM (CD326) (orange) and CD31 (green). Scale bars, 150, 80, and 40 μm. (B) Schematic equation illustrating normalization of cytokine production from combination of MVN and H1355 tumor spheroids. Cytokine production is considered supra-additive (>1), additive (=1) or antagonistic (<1). (C) Heat map of log2 fold change cytokine/chemokine profiles in conditioned medium after 7 days of 3D microfluidic culture of MVN with H1355 LUC and LKB1 spheroids pre-stimulated with 1 μg/mL poly (dA:dT). (D) Absolute values of supra-additive cytokine release of human CCL2, G-CSF, CXCL10, and IL-6, from 3D microfluidic culture of combination of MVN + spheroids of H1355 LKB1 and LUC control spheroids and MVN only after 7 days of culture in 3D microfluidic devices. P values were calculated by one-way ANOVA followed by Tukey post hoc test; **P < 0.01. Data shown as mean values, error bars ± SD.