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. 2020 Jul 9;21(9):e50308. doi: 10.15252/embr.202050308

Figure EV5. USP44 stabilizes FOXP3 in a dose‐dependent manner.

Figure EV5

HEK293T cells were transfected with an expression construct encoding MYC‐FOXP3 (0.5 μg), and varying levels (0.5, 1, 1.5 μg) of vectors encoding FLAG‐tagged wild‐type USP44 (wt) or the mutant (Kralovics et al, 2005), after 48 h transfected cells were harvested and lysed in RIPA buffer. Immunoblots were analyzed using anti‐FLAG or anti‐MYC antibodies. Shown is a representative result of three independent experiments (biological replicates). Source data are available online for this figure.