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. 2020 Jul 5;8(9):e1390. doi: 10.1002/mgg3.1390

Figure 2.

Figure 2

PCR mapping in F8 intron 1. (a) Primer design of mapping PCR in F8 intron 1. F8 intron 1 is divided into four segments, Seg 1 (4.8 kb), Seg 2 (8.2 kb), Seg 3 (4.9 kb), and Seg 4 (6.3 kb). (b) Results of F8 intron 1 mapping in normal male individual (left) and proband (right). Segments 2 and 3 were not detected in the proband's gDNA. (c) Full‐length PCR amplification of F8 intron 1. Long‐range PCR successfully amplified the product in the gDNA of normal male subject (N), while no amplicon was detected in the proband's gDNA (P)