Figure 3.

ASMTL‐AS1 was transcriptionally enhanced by MYC. A, Transcription factors for ASMTL‐AS1 were predicted by UCSC and PROMO. B, Relative expression of three potential transcription factors in Huh7 and Huh7‐H cells was tested through qRT‐PCR. C, D, qRT‐PCR results of MYC in HCC cell lines (C) or in indicated HCC tissues (D). E, Pearson's correlation analysis determined the association between MYC and ASMTL‐AS1 in clinical tissues from HCC patients with or without RFA treatment. F, G, qRT‐PCR analysis of the expression levels of MYC or ASMTL‐AS1 in MYC‐overexpressed Huh7 cells or in MYC‐inhibited Huh7‐H cells. H, I, The interaction of MYC with ASMTL‐AS1 promoter and its impact on ASMTL‐AS1 transcription were evaluated via ChIP and luciferase reporter assays, respectively. J, K, ChIP and luciferase reporter assays further confirmed the précised binding sequences for MYC to activate ASMTL‐AS1 transcription. *P < .05, **P < .01