Figure 5.

ASMTL‐AS1 regulated HCC malignancy via NLK‐activated YAP signalling. A, qRT‐PCR revealed ASMTL‐AS1 had no impact on the mRNA expression of YAP. B, The protein expression of NLK, YAP and p‐YAP at Ser¹²⁷ or Ser¹²⁸ in ASMTL‐AS1‐overexpressed Huh7 cells or ASMTL‐AS1‐depleted Huh7‐H cells was tested by Western blotting. C, Influence of ASMTL‐AS1 on the interaction of YAP with NLK or 14‐3‐3 was assayed via Co‐IP. D, E, Impact of ASMTL‐AS1 on YAP translocation was assessed by subcellular fractionation plus Western blot (D) and IF assay (E; scale bar = 20 μm). F, The mRNA or protein expression of NLK in indicated cells was analysed by qRT‐PCR or Western blotting, respectively. G, Rescue assays indicated that NLK knockdown recovered ASMTL‐AS1 overexpression‐accelerated proliferation and motility in Huh7 cells. H, I, Rescue assays proved overexpression of NLK countervailed the inhibitory effect of ASMTL‐AS1 depletion on the malignant behaviours of Huh7‐H cells. **P < .01