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. 2020 Sep 8;14:909. doi: 10.3389/fnins.2020.00909

FIGURE 2.

FIGURE 2

Sleep restriction compromised leptin receptor signaling via JAK2/STAT3 in the hypothalamus. (A) Western blot analysis. Images were the representative results of five independent experiments for each group. (B) Densitometric analysis of Western blots for LepRb. (C) Leptin levels in the serum. (D) Densitometric analysis of Western blots for p-JAK2, JAK2, and the p-JAK2/JAK2 ratio. (E) Densitometric analysis of Western blots for p-STAT3, STAT3, the p-STAT3/STAT3 ratio, SOCS3, and POMC. The relative intensity in arbitrary units compared to β-actin and presented as fold change vs. control sample. (F) Quantitation of LepRb, JAK2, STAT3, SOCS3, and POMC mRNA by real-time RT-PCR. Gene expression was normalized to GAPDH and presented as a fold change vs. the control. Data were expressed as the mean ± SD of six experiments and analyzed by independent-sample t-test. Significant difference was defined as *p < 0.05 vs. control group. SR, sleep restriction group; CON, control group.