TABLE 1.

Oligonucleotide sequences used for real-time RT-PCR.

Gene	Primer sequences	Product (bp)
LepRb	5′-TCTTCTGGAGCCTGAACCCATTTC-3′	675
	5′-TTCTCACCAGAGGTCCCTAAACT-3′
JAK2	5′-TTTGAAGACAGGGACCCTACACAG-3′	101
	5′- TCATAGCGGCACATCTCCACA-3′
STAT3	5′-CACCCATAGTGAGCCCTTGGA-3′	137
	5′-TGAGTGCAGTGACCAGGACAGA-3′
POMC	5′-CATAGACGTGTGGAGCTGGT-3′	149
	5′-TCAAGGGCTGTTCATCTCCG-3′
SOCS3	5′-AGTCGGGGACCAAGAACCTA-3′	172
	5′-GAAGGTTCCGTCGGTGGTAA-3′
IRS1	5′-AGAGTGGTGGAGTTGAGTTG-3′	277
	5′-GGTGTAACAGAAGCAGAAGC-3′
IRS2	5′-GACTTCTTGTCCCATCACTTGAAA-3′	112
	5′-GCTAAGCATCTCCTCAGAATGGA-3′
PI3K	5′-GCCCAGGCTTACTACAGAC-3′	248
	5′-AAGTAGGGAGGCATCTCG-3′
AKT	5′-CAAGATGACAGCATGGAGTGTG-3′	189
	5′-CCAGCACATCCGAGAAACAAAA-3′
mTOR	5′-GACAACAGCCAGGGCCGCAT-3′	154
	5′-ACGCTGCCTTTCTCGACGGC-3′
FoxO1	5′-TCCTCGAACCAGCTCAAACG-3′	288
	5′-GGCGGTGCAAATGAATAGCAAG-3′
CLOCK	5′-CTGTTACATCAGCACGCCTC-3′	145
	5′-TACTGCTTGGCTCTTCTGCT-3′
BMAL1	5′-CCGATGACGAACTGAAACACC-3′	78
	5′-TCTTCCCTCGGTCACATCCT-3′
CRY1	5′-ACGTGATAGGGAAGTGCACA-3′	135
	5′-GTGCCTCAGTTTCTCCTCCT-3′
CRY2	5′-ACCGCCTGTGGGACTTGTA-3′	80
	5′-TCGCCATAGGAGTTGTCCAAATA-3′
PER1	5′-GCTCCTGACCAAGCCTCATT-3′	165
	5′-GCAGGAAGAGGAGGCACATT-3′
PER2	5′-CTGCGAAGCGCCTCATTCC-3′	295
	5′-TTATGCTCCGCCTCTGTCATC-3′
GAPDH	5′-GCAAGAGAGAGGCCCTCAG-3′	74
	5′-TGTGAGGGAGATGCTCAGTG-3′