FIGURE 3.

Effect of rAPN on LPS‐induced cytokine expression in PASMCs. To determine the effect of rAPN on LPS‐induced expression in PASMCs during development, PASMCs from PND4 and PND7 rat pups were treated with rAPN (20 µg/ml) or with LPS (3 µg/ml) + rAPN (20 µg/ml) (a–c) in culture for 48 hr (n = 4 per group); afterward the cells were homogenized and protein extracted from western blot (WB). Immunoblots were normalized to β‐actin. Bars represent mean ± SEM.*p < .05 by one‐way ANOVA followed by post hoc analyses using Holm–Sidak test compared to all other groups; #p < .05 compared to corresponding control groups. This experiment was repeated four times