FIGURE 5.

Effect of rAPN on the expression of lung mRNA and protein levels for inflammatory chemokines in response to aspiration of LPS, in vivo. PND4 pups were treated with rAPN (2 mg/kg) or saline by IP injections, and after 4 hr, LPS (5 mg/kg) or saline were administered by IPh route. After 24 hr, lungs were collected and processed for qPCR and western blot (WB). mRNA relative expression of MCP‐1 and MIP‐1α (a, c); and protein content of MCP‐1, MIP‐1α, and IL‐8 (b, d, e) was measured (n = 4 per group). mRNA relative expression change was calculated by 2ΔCt method normalized to β‐actin mRNA expression. Immunoblots were normalized to β‐actin and representative immunoblots under each graph show two contiguous lanes for each group. Bars represent mean ± SEM by one‐way ANOVA followed by post hoc analyses using Holm–Sidak test. * represents p < .05 compared to all other groups and # represents p < .05 compared to saline IP + LPS IPh group