FIGURE 9.

Effect of rAPN given after aspiration of LPS on mRNA and protein expression for inflammatory cytokines, in vivo, rescue. PND4 rats were given LPS (5 mg/kg) or saline IPh followed by rAPN (2 mg/kg) or saline by IP injections 20 hr after LPS. (n = 4 per group). Six hours after rAPN and 24 hr after LPS was given, lungs were removed and processed for mRNA (left panel) or protein (right panel) detection for IL‐6 (a and b), TNF‐α (c and d), IL‐1β (e and f), and IL‐10 (g and h). Change in relative mRNA expression was calculated by 2ΔCt method normalized to β‐actin mRNA expression. Immunoblots were normalized to β‐actin protein expression. Bars represent mean ± SEM. One‐way ANOVA followed by post hoc analyses using Holm–Sidak test *p < .05 compared to other groups; ¥ represents p < .05 compared to saline/saline and LPS/rAPN treatment groups for TNF‐α and IL‐10; # represents p < .05 compared to saline/saline and LPS/rAPN treatment groups for IL‐1β