FIGURE 10.

Effect of the rAPN when given as rescue treatment on LPS‐induced lung chemokine production, in vivo. PND4 rats were given LPS (5 mg/kg) or saline IPh followed by rAPN (2 mg/kg) or saline by IP injections 20 hr after LPS (n = 4 per group). Six hours after rAPN was given, lungs were removed and processed for mRNA (left panel) or protein (right panel) detection of MCP‐1 (a and b) and MIP‐1α (c and d). Change in relative mRNA expression was calculated by 2ΔCt method normalized to β‐actin mRNA expression. Immunoblots were normalized to β‐actin protein expression. Bars represent mean ± SEM. One‐way ANOVA followed by post hoc analyses using Holm–Sidak test. * represents p < .05 compared to other groups