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. 2020 Jul 9;39(18):e104494. doi: 10.15252/embj.2020104494

Figure EV1. Infection triggers LRRK2‐dependent Rab8A phosphorylation in macrophages.

Figure EV1

  • A–C
    RAW264.7 macrophages were left uninfected or infected with (A) Mycobacterium tuberculosis (Mtb), (B) C. albicans (Ca) or (C) L. monocytogenes (Lm) for the indicated time, and Rab8A pT72, Rab10 pT73 and LRRK2 pS935 levels were analysed by Western blot.
  • D–F
    WT and LRRK2 KO macrophages were left uninfected or infected with (D) Mtb for 24 h, (E) Ca or (F) Lm for 120 min. Rab8A pT72 and LRRK2 pS935 phosphorylation was analysed by Western blot.
  • G–I
    RAW264.7 macrophages were pre‐treated with 1 μM GSK2578215A (GSK inh) or 0.1 μM MLi‐2 and left uninfected or infected with (G) Mtb for 24 h, (H) Ca or (I) Lm for 120 min. Rab8A pT72 and LRRK2 pS935 phosphorylation was analysed by Western blot and quantified by densitometry. Beta‐actin was used as loading control. Data represent the mean + SEM of three independent biological replicates. One‐way ANOVA followed by Dunnett's test against DMSO control. ns = non‐significant; *P < 0.05; ***P < 0.001

Source data are available online for this figure.