KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| mouse anti-Bruchpilot (nc82) | Developmental Studies Hybridoma Bank | Cat# nc82; RRID: AB_2314866 |
| chicken anti-GFP | Abcam | Cat# ab13970; RRID:AB_300798 |
| rabbit-anti DsRed (Living Colors) | Takara Bio | Cat# 632496; RRID: AB_10013483 |
| rat mAb anti-FLAG | Novus Biologicals | Cat# NBP1-06712B; RRID: AB_10006034 |
| Rabbit anti-HA | Cell Signaling Technologies | Cat# 3724; RRID:AB_1549585 |
| rabbit anti-GABA | Sigma-Aldrich | Cat# A2052; RRID: AB_477652 |
| DyLight 500-conjugated mouse anti-V5 | Bio-Rad | Cat# MCA1360D550GA; RRID: AB_2687576 |
| Alexa Fluor 405-conjugated goat anti-mouse | Thermo Fisher Scientific | Cat# A-31553, RRID:AB_221604 |
| Alexa Fluor 488-conjugated goat anti-chicken | Thermo Fisher Scientific | Cat# A-11039; RRID: AB_2534096 |
| Alexa Fluor 488-conjugated goat anti-rabbit | Thermo Fisher Scientific | Cat# A-11008; RRID: AB_143165 |
| Alexa Fluor 568-conjugated goat anti-rabbit | Thermo Fisher Scientific | Cat# A-11011; RRID: AB_143157 |
| Alexa Fluor 594-conjugated donkey anti-rabbit | Jackson ImmunoResearch Laboratories | Cat# 711-585-152; RRID:AB_2340621 |
| Alexa Fluor 633-conjugated goat anti-mouse | Thermo Fisher Scientific | Cat# A-21050; RRID: AB_2535718 |
| ATTO-647-conjugated goat anti-rat | Rockland | Cat# 612-156-120; RRID: AB_10893386 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Streptavidin, Alexa Fluor 568 conjugate | Thermo Fisher Scientific | Cat# S11226; RRID: AB_2315774 |
| Streptavidin, Alexa Fluor 633 conjugate | Thermo Fisher Scientific | Cat# S21375; RRID: AB_2313500 |
| Tetrodotoxin (TTX) | Enzo | BML-NA120-0001 |
| Tetrodotoxin citrate | Abcam | ab120055 |
| Picrotoxin | Sigma-Aldrich | P1675 |
| Vectashield mounting medium | Vector Laboratories | Cat# H-1000, RRID:AB_2336789 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: Dickinson wild type | Michael Dickinson lab | N/A |
| D. melanogaster: P{pBDPGAL4.1Uw}attP2 | Bloomington Drosophila Stock Center (BDSC) | RRID:BDSC_68384 |
| D. melanogaster: P{R12G08-Gal4}attP2 | BDSC | RRID:BDSC_47855 |
| D. melanogaster: P{R20A02-Gal4}attP2 | BDSC | RRID:BDSC_48870 |
| D. melanogaster: P{R26B07-Gal4}attP2 | BDSC | RRID:BDSC_49160 |
| D. melanogaster: P{R31A12-Gal4}attP2 | BDSC | RRID:BDSC_49661 |
| D. melanogaster: P{R54E12-Gal4}attP2 | BDSC | RRID:BDSC_39077 |
| D. melanogaster: P{R60D05-Gal4}attP2 | BDSC | RRID:BDSC_39247 |
| D. melanogaster: P{VT017183-Gal4}attP2 | Vienna Drosophila Resource Center (VDRC) | RRID:FlyBase_FBst0484665 |
| D. melanogaster: P{VT040354-Gal4}attP2 | VDRC | RRID:FlyBase_FBst0487578 |
| D. melanogaster: P{VT059225-p65.AD}attP40 | BDSC | RRID:BDSC_71395 |
| D. melanogaster: P{VT048577-GAL4.DBD}attP2 | BDSC | RRID:BDSC_72828 |
| D. melanogaster: P{repo-Gal80} | Tzumin Lee lab | N/A |
| D. melanogaster: P{R53B02-LexA}attP40 | BDSC | RRID:BDSC_53615 |
| D. melanogaster: P{R60D05-LexA}attP40 | BDSC | RRID:BDSC_52867 |
| D. melanogaster: P{Or83b-LexA} | Tzumin Lee lab | N/A |
| D. melanogaster: P{20XUAS-GCaMP6f}attP40 | BDSC | RRID:BDSC_42747 |
| D. melanogaster: Pbac{20XUAS-IVS-jGCaMP7f}VK00005 | BDSC | RRID:BDSC_79031 |
| D. melanogaster: P{UAS-DenMark}3, P{UAS-syt.eGFP}3 | BDSC | RRID:BDSC_33065 |
| D. melanogaster: P{UAS-Hsap\KCNJ2.EGFP}7 | BDSC | RRID:BDSC_6595 |
| D. melanogaster: P{20×UAS-CsChrimson-mCherry-trafficked}su(Hw)attP1 | Vivek Jayaraman lab | N/A |
| D. melanogaster: P{20XUAS-GtACR1-EYFP}attP2 | Adam Claridge-Chang lab | N/A |
| D. melanogaster: P{20XUAS-IVS-mCD8::GFP}attP40 | Gerry Rubin lab | RRID:BDSC_32194 |
| D. melanogaster: P{10XUAS-IVS-mCD8::GFP}su(Hw)attP8 | BDSC | RRID:BDSC_32189 |
| D. melanogaster: P{13XLexAop2-IVS-GCaMP6f-p10}su(Hw)attP5 | BDSC | RRID:BDSC_44277 |
| D. melanogaster: P{13XLexAop2-IVS-myr::GFP}VK00005 | Gerry Rubin lab | RRID:BDSC_32209 |
| D. melanogaster: P{13XLexAop2-IVS-myr::GFP}su(Hw)attP1 | BDSC | RRID:BDSC_32212 |
| D. melanogaster: P{13XLexAop2-IVS-CsChrimson.mVenus}attP18 | BDSC | RRID:BDSC_55137 |
| D. melanogaster: MCFO-3 | BDSC | RRID:BDSC_64087 |
| D. melanogaster: MCFO-4 | BDSC | RRID:BDSC_64088 |
| D. melanogaster: MCFO-5 | BDSC | RRID:BDSC_64089 |
| Software and Algorithms | ||
| MATLAB | MathWorks | RRID:SCR_001622 |
| ScanImage | Pologruto et al., 2003 | RRID:SCR_014307 |
| LEAP (LEAP Estimates Animal Pose) | https://github.com/talmo/leap | Pereira et al., 2018 |
| Constrained nonnegative matrix factorization for calcium imaging data | https://github.com/flatironinstitute/CaImAn-MATLAB | Pnevmatikakis et al., 2016 |
| FicTrac v2 | http://rjdmoore.net/fictrac/ | Moore et al., 2014 |
| Fiji | http://fiji.sc | RRID:SCR_002285 |