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. Author manuscript; available in PMC: 2020 Sep 22.
Published in final edited form as: ACS Chem Biol. 2020 Jun 12;15(7):1773–1779. doi: 10.1021/acschembio.0c00205

Figure 1. mRNA photo-caging using the RNA-TAG technique.

Figure 1.

A) To facilitate TGT enzymatic labeling, three enzyme recognition sequences, ‘Tags’, are genetically inserted along the 5’-UTR of an IVT-mRNA. Subsequent conjugation of the ‘photo-cages’ severely reduces mRNA translation activity. B) Chemical structures of two sequentially activable preQ1 derivatives (‘photo-cages’), biotin-Bac-preQ1 and biotin-DEACM-preQ1.