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. Author manuscript; available in PMC: 2020 Sep 22.
Published in final edited form as: ACS Chem Biol. 2020 Jun 12;15(7):1773–1779. doi: 10.1021/acschembio.0c00205

Figure 2. TGT labeling of the RNA ‘Tag’ oligo and in-vitro sequential photo-uncaging.

Figure 2.

A) In-vitro conjugation of RNA ‘Tag’ oligo with preQ1 derivatives through RNA-TAG. B) Denaturing 18% PAGE analysis of enzymatic labeling and subsequent photo-uncaging of the RNA ‘Tag’ oligos. Compared to the unlabeled RNA oligo, an upper gel shift in the second column demonstrates covalent attachment of the ‘photo-cage’ onto the RNA oligo. Lower gel shift in the third or fourth column demonstrates photo-release of the ‘photo-cage’ from the RNA oligo. After photo-cleavage, the resulting RNA band was only slightly up-shifted compared to the starting material, supporting that only a minimal residue was left on the RNA after photo-cleavage.