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. 2020 Sep 4;3(5):1008–1016. doi: 10.1021/acsptsci.0c00108

Figure 1.

Figure 1

Schematic representation of the fluorogenic SARS-CoV-2 protease enzymatic assay. The peptide substrate exhibits low fluorescent because the fluorescence intensity of Edans in the C-terminal is quenched by the Dabcyl in the N-terminal of the substrate. The protease cleaves the substrate which breaks the proximity of the quencher molecule Dabcyl with the fluorophore Edans, resulting in an increase in fluorescence signal. This increase in fluorescence signal is proportional to the protease activity.