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. 2020 Sep 21;12:142. doi: 10.1186/s13148-020-00937-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — DNA methylation analysis of DAC-treated HCT116 cells at the single-cell level. a Experimental protocol of the single cell cloning of DAC-treated HCT116 cells. Single cells were isolated by the limiting dilution method, and nine clones were obtained. b Changes of DNA methylation levels in DAC-treated bulk cells. Only partial demethylation was induced by DAC treatment. c Changes of DNA methylation levels in DAC-treated clones. Methylation levels were markedly reduced by DAC treatment, and 12.1–28.2% of TSS200CGIs methylated in HCT116 cells were completely demethylated