Figure 2: Confirmation of UV induced apoptosis in Jurkat T cells.

Jurkat T cells were exposed to UV (60 mJ/cm²) using a UV Crosslinker (Model 1800). Following UV exposure, Jurkat T cells were incubated at 37 °C with 5% CO₂ for 4 h. Following incubation, Jurkat T cells were stained with annexin V and propidium iodide (PI), and apoptosis was evaluated by flow cytometry. Early apoptotic, late apoptotic, and necrotic cells are identified as annexin V⁺/PI−, annexin V⁺/PI⁺, annexin V⁻PI⁺, respectively. Representative flow cytometry scatter plots (with 10,000 events recorded) of (A) unexposed Jurkat T cells and (B) UV-exposed Jurkat T cells.