Skip to main content
. Author manuscript; available in PMC: 2020 Sep 22.
Published in final edited form as: J Vis Exp. 2019 Oct 22;(152):10.3791/60109. doi: 10.3791/60109

Figure 3: O3 exposure decreases alveolar macrophage efferocytosis.

Figure 3:

C57BL/6J female mice were exposed to filtered air (FA) or 1 ppm O3for 3 h. 24 h post-exposure, mice were oropharyngeally instilled with approximately 5 x 106 apoptotic Jurkat T cells. 1.5 h after instillation, bronchoalveolar lavage (BAL) was performed, and the efferocytic index was calculated in BAL macrophages by light microscopy after counting 200 macrophages (n = 11 per group). (A) Representative image of an efferocytic macrophage. (B) Identification of alveolar macrophages (white arrows) and efferocytic macrophage (black arrows) after FA or O3 exposure. (C) Calculation of the efferocytic index after FA or O3 exposure (***p < 0.0001).