Figure 1.

Differential gene expression between primary tumors and lymph nodes shows CXCL14 as the top downregulated gene in metastases. Volcano plot shows differentially expressed genes in five primary tumor samples relative to matched metastatic cervical lymph nodes, in malignant cells profiled by single cell RNA-sequencing. Genes are plotted based on differential expression (x-axis) and significance of this difference (y-axis). They are also colored based on the number of individual patient samples in which differential expression was significant. CXCL14 was the most significantly downregulated gene across lymph node samples and demonstrated significant downregulation in three individual patients.