Figure 4.

Modulation of CXCL14 is associated with direct changes in tumor infiltrating lymphocytes (TIL). (A) Bar plot shows significant decrease in absolute number of CD45⁺CD3⁺ cells (cells/1×10⁵) in mouse oral cavity (MOC)1-derived tumor after CXCL14 shRNA knockdown (n=3 biological replicates, 2 independent experiments, p=0.02, error bars represent SEM). (B) Bar plot shows significant increase in absolute number of CD45⁺CD3⁺ cells (cells/1×10⁵) in MOC2-derived tumor after CXCL14 overexpression (n=3 biological replicates, 2 independent experiments, p=0.006, error bars represent SEM). (C) Fluorescent activated cell sorting (FACS) plots show decrease in proportion of intratumoral CD45⁺CD3⁺ T cells from 22% in control MOC1-derived tumor (left panel) to 9% after CXCL14 shRNA knockdown (right panel). (D) FACS plots show increase in proportion of intratumoral CD45⁺CD3⁺ T cells from 13% in control MOC2-derived tumor (left panel) to 21% after CXCL14 overexpression (right panel).