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. 2020 Sep 21;8(2):e001224. doi: 10.1136/jitc-2020-001224

Figure 5.

Figure 5

Poly-IC stabilized with poly-lysine and carboxymethylcellulose (PICLC) induces the adhesion molecules and chemokine expression in vascular endothelial cells (VECs). (A) In vitro induction of CXCL10 in mouse vascular endothelial cells. Mouse VECs (bEnd.3) were stimulated with PICLC or interferon-β (IFN-β), and 24 hours later, CXCL10 levels in the supernatants were assessed by ELISA. (B) VCAM-I and CXCL9 expression in tumor and normal (lungs and hearts) VECs. Wild-type (WT) mice were inoculated subcutaneously with 5X105 B16F10 cells and received intravenous administration of phosphate-buffered saline (PBS) (No Tx), 50 μg of Poly-IC or 50 μg of PICLC on days 10 and 12. On day 13, the levels of VCAM-I and CXCL9 in VECs from normal tissues (lungs and hearts) and tumors were analyzed by flow cytometry in the CD45−, CD31+ population. Mean fluorescence intensity (MFI) values for each condition are shown. Data are pooled from three mice per group. Statistical differences between treatment groups were calculated using a univariate χ2 test within the FlowJo software. **P=0.000151; ***p=0.000231; ****p=8.38X10-8. (C, D) IFN-I production, VCAM-I and CXCL9 expression in human endothelial cells. Human vascular endothelial cells (HUVECs) were stimulated Poly-AU, Poly-IC or PICLC for 24 hours and levels of secreted IFN-I (C) and expression of VCAM-I and CXCL9 were measured by flow cytometry (D).