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. Author manuscript; available in PMC: 2020 Sep 22.
Published in final edited form as: J Vis Exp. 2019 Sep 17;(151):10.3791/60242. doi: 10.3791/60242

Table of Materials

Name of Material/ Equipment Company Catalog Number Comments/Description
Absolutely RNA Miniprep Kit Agilent 400800
Anti-GFP antibodies Memorial Sloan-Kettering Antibody & Bioresource Core GFP Ab #19C8 and GFP Ab #19F7
Bovine Serum Albumin, IgG-Free, Protease-Free Jackson ImmunoResearch 001-000-162
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Roche 11836170001
Cycloheximide Millipore Sigma C7698
D-Glucose, Dextrose Fisher Scientific D16
Deoxycholic acid, DOC Millipore Sigma D2510
DL-Dithiothreitol Millipore Sigma D9779
Dynabeads MyOne Streptavidin T1 Thermo Fisher Scientific 65602
HBSS (10X), calcium, magnesium, no phenol red Thermo Fisher Scientific 14065–056
HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure, Thermo Scientific Thermo Fisher Scientific AAJ16924AE
Magnesium chloride, MgCl2 Millipore Sigma M8266
Methanol Fisher Scientific A452
NEBNext Poly(A) mRNA Magnetic Isolation Module New England BioLabs E7490S
NEBNext Ultra RNA Library Prep Kit for Illumina New England BioLabs E7530S
Nonylphenyl polyethylene glycol, NP-40. IGEPAL CA-630 Millipore Sigma I8896
Nuclease-Free Water, not DEPC-Treated Ambion AM9932
PBS Buffer (10X), pH 7.4 Ambion AM9625
Pierce Recombinant Protein L, Biotinylated Thermo Fisher Scientific 29997
Potassium chloride, KCl Millipore Sigma P4504
RNaseZap RNase Decontamination Solution Invitrogen AM9780
RNasin Ribonuclease Inhibitors Promega N2515
RNA 6000 Pico Kit & Reagents Agilent 5067–1513
Sodium azide, NaN3 Millipore Sigma S2002
Sodium bicarbonate, NaHCO3 Millipore Sigma S6297
SUPERase·In RNase Inhibitor Invitrogen AM2694
Overhead Stirrer DWK Life Sciences (Wheaton) 903475
10 ml Tissue Grinder, Potter-Elv, Coated DWK Life Sciences (Wheaton) 358007