Absolutely RNA Miniprep Kit |
Agilent |
400800 |
|
Anti-GFP antibodies |
Memorial Sloan-Kettering Antibody & Bioresource Core |
GFP Ab #19C8 and GFP Ab #19F7 |
Bovine Serum Albumin, IgG-Free, Protease-Free |
Jackson ImmunoResearch |
001-000-162 |
|
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail |
Roche |
11836170001 |
|
Cycloheximide |
Millipore Sigma |
C7698 |
|
D-Glucose, Dextrose |
Fisher Scientific |
D16 |
|
Deoxycholic acid, DOC |
Millipore Sigma |
D2510 |
|
DL-Dithiothreitol |
Millipore Sigma |
D9779 |
|
Dynabeads MyOne Streptavidin T1 |
Thermo Fisher Scientific |
65602 |
|
HBSS (10X), calcium, magnesium, no phenol red |
Thermo Fisher Scientific |
14065–056 |
|
HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure, Thermo Scientific |
Thermo Fisher Scientific |
AAJ16924AE |
|
Magnesium chloride, MgCl2 |
Millipore Sigma |
M8266 |
|
Methanol |
Fisher Scientific |
A452 |
|
NEBNext Poly(A) mRNA Magnetic Isolation Module |
New England BioLabs |
E7490S |
|
NEBNext Ultra RNA Library Prep Kit for Illumina |
New England BioLabs |
E7530S |
|
Nonylphenyl polyethylene glycol, NP-40. IGEPAL CA-630 |
Millipore Sigma |
I8896 |
|
Nuclease-Free Water, not DEPC-Treated |
Ambion |
AM9932 |
|
PBS Buffer (10X), pH 7.4 |
Ambion |
AM9625 |
|
Pierce Recombinant Protein L, Biotinylated |
Thermo Fisher Scientific |
29997 |
|
Potassium chloride, KCl |
Millipore Sigma |
P4504 |
|
RNaseZap RNase Decontamination Solution |
Invitrogen |
AM9780 |
|
RNasin Ribonuclease Inhibitors |
Promega |
N2515 |
|
RNA 6000 Pico Kit & Reagents |
Agilent |
5067–1513 |
|
Sodium azide, NaN3 |
Millipore Sigma |
S2002 |
|
Sodium bicarbonate, NaHCO3 |
Millipore Sigma |
S6297 |
|
SUPERase·In RNase Inhibitor |
Invitrogen |
AM2694 |
|
Overhead Stirrer |
DWK Life Sciences (Wheaton) |
903475 |
|
10 ml Tissue Grinder, Potter-Elv, Coated |
DWK Life Sciences (Wheaton) |
358007 |
|