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. 2020 Sep 22;16(9):e9443. doi: 10.15252/msb.20209443

Figure EV4. CRISPR Cas9‐based knockout of PYCR1 in MDA‐MB‐231 cells. Related to Fig 6.

Figure EV4

  1. Western blots showing PYCR1 knockout in MDA‐MB-231 cells. Bar plot shows quantitative analysis of the Western blot.
  2. Representative pictures of Transwell migration and invasion after PYCR1 knockout. Scale bar: 100 μm.
  3. Bar plots represent mean ± SD of three biological replicates of the invasion and migration assays. Samples are compared using paired Student's t‐test. P values are indicated as ***P < 0.001.
  4. Growth measurements of MDA‐MB-231 wild‐type and PYCR1 KO cells over 96 h. Data represent mean ± SE of three biological replicates. Samples are compared using paired Student's t‐test. P values are indicated as follows *P < 0.05 and **P < 0.01.
  5. Bar plots show percentage of viable cells after treatment with 0.312 μM doxorubicin and 1.25 μM paclitaxel. Data represent mean ± SE of three biological experiments. Samples are compared using Student's t‐test.
  6. Tumor volume measurements for 26 days in MDA‐MB-231‐injected NSG mice. CRISPR control (n = 6) and PYCR1 KO tumors (n = 6) without treatment are shown. Data represent mean ± SE. Samples on day 26 are compared using paired Student's t‐test, and P values are reported as follows **P < 0.01.
  7. Bar plot indicates mean ± SE of tumor weight measurements for day 26. CRISPR control (n = 6) and PYCR1 KO tumors (n = 6) without treatment are shown. Samples are compared using paired Student's t‐test. P values are reported as **** P < 0.0001.
  8. Picture of excised tumors on day 26.