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. 2020 Sep 22;1(6):100095. doi: 10.1016/j.xcrm.2020.100095

Figure 2.

Figure 2

Effector CD4 T Cell Response to Adjuvanted Vaccines

Groups of C57BL/6 mice were vaccinated IN, as in Figure 1. At day 8 PV, cells from lungs and BAL were stained with I-Ab/NP311 tetramers along with antibodies to cell surface molecules and transcription factors.

(A) FACS plots show the percentages of I-Ab/NP311 tetramer-binding cells among CD4 T cells.

(B) Percentages of the indicated cell population among NP311-specific, tetramer-binding CD4 T cells.

(C) FACS plots are gated on I-Ab/NP311 tetramer-binding cells, and the numbers in each quadrant are the percentages of cells among the gated population; MFIs for transcription factors in NP311-specific CD4 T cells are plotted in the adjoining graphs.

(D) FACS plots in (C) were used to quantify the percentages of T-betLOEOMESHI cells (quadrant 4) among NP311-specific CD4 T cells.

(E) Percentages of CD103HI and CD69HI cells among NP311-specific CD4 T cells.

Data are representative of two independent experiments. Comparisons were made using one-way ANOVA test with Tukey-corrected multiple comparisons; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.