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. 2020 Sep 22;1(6):100095. doi: 10.1016/j.xcrm.2020.100095

Figure 5.

Figure 5

Mucosal CD8 and CD4 T Cell Memory in Vaccinated Mice

At 100 days after booster vaccination, NP366-specific memory CD8 T cells (A–D) and NP311-specific CD4 T cells (E–H) were characterized in lungs, airways (BAL), and spleen. To stain for vascular cells, mice were injected intravenously with fluorescent-labeled anti-CD45.2 antibodies, 3 min prior to euthanasia. Cells from lungs and BAL were stained with Db/NP366 tetramers, I-Ab/NP311 tetramers, and anti-CD4, anti-CD8, anti-CD44, anti-CD103, and anti-CD69 antibodies.

(A) Percentages and total numbers of NP366-specific CD8 T cells in lungs, BAL, and spleen.

(B) FACS plots are gated on NP366-specific, tetramer-binding CD8 T cells; numbers are the percentages of vascular and non-vascular cells in the gated population.

(C) Percentages of CD69+veCD103+ve TRM cells among NP366-specific CD8 T cells.

(D) Total numbers of vascular and non-vascular CD103+ve NP366-specific CD8 T cells in lungs.

(E) Percentages and total numbers of NP311-specific CD4 T cells in lungs.

(F) Percentages of vascular and non-vascular cells among NP311-specific CD4 T cells in lungs.

(G) Percentages of IFN-γ- or IL-17-producing cells among CD4 T cells.

(H) Calculated percentages of IFN-γ- and/or IL-17-producing CD4 T cells among total NP311-specific, cytokine-producing (IFN-γ + IL-17) peptide-stimulated CD4 T cells.

Data are pooled from two independent experiments. Comparisons were made using one-way ANOVA test with Tukey-corrected multiple comparisons; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.