Table 1.
Bacteria | Microdilution Antibiotic Positive Controls (ug/ml) | Microdilution Negative Controls |
---|---|---|
Gram negative bacteria | Blank: 100 μl CAMHB Growth control: 50 μl CAMHB: 50 μl inoculum 1% DMSO growth control: 1 μl DMSO in 50 μl CAMHB: 50 μl inoculum 2% DMSO growth control: 2 μl DMSO in 50 μl CAMHB: 50 μl inoculum Solvent growth control: 8.7 μl acetic acid: acetonitrile: water (1:2:7) in 50 μl CAMHB: 50 μl inoculum |
|
Enterobacter cloacae | Ampicillin 128, 64, 32, 16, 8, 4, 2, 1, 0.5, and 0.25 | |
Klebsiella oxytoca | ||
Escherichia coli | Ampicillin 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625 | |
Salmonella typhimurium | ||
Pseudomonas aeruginosa | Gentamicin 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625 | |
Gram positive bacteria | ||
Enterococcus faecalis | Ampicillin 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625 | |
Staphylococcus saprophyticus | Ampicillin 4, 2, 1, 0.5, 0.25, 0.125, 0.0625, 0.031, 0.016, and 0.008 | |
Staphylococcus aureus | ||
Streptococcus pyogenes |
CAMHB, Cation-Adjusted Mueller-Hinton broth.