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. 2020 Sep 8;11:1340. doi: 10.3389/fphar.2020.01340

Table 1.

List of bacteria and controls used in the antimicrobial susceptibility tests.

Bacteria Microdilution Antibiotic Positive Controls (ug/ml) Microdilution Negative Controls
Gram negative bacteria Blank: 100 μl CAMHB
Growth control: 50 μl CAMHB: 50 μl inoculum
1% DMSO growth control: 1 μl DMSO in 50 μl CAMHB: 50 μl inoculum
2% DMSO growth control: 2 μl DMSO in 50 μl CAMHB: 50 μl inoculum
Solvent growth control: 8.7 μl acetic acid: acetonitrile: water (1:2:7) in 50 μl CAMHB: 50 μl inoculum
Enterobacter cloacae Ampicillin 128, 64, 32, 16, 8, 4, 2, 1, 0.5, and 0.25
Klebsiella oxytoca
Escherichia coli Ampicillin 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625
Salmonella typhimurium
Pseudomonas aeruginosa Gentamicin 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625
Gram positive bacteria
Enterococcus faecalis Ampicillin 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625
Staphylococcus saprophyticus Ampicillin 4, 2, 1, 0.5, 0.25, 0.125, 0.0625, 0.031, 0.016, and 0.008
Staphylococcus aureus
Streptococcus pyogenes

CAMHB, Cation-Adjusted Mueller-Hinton broth.