Table 4.
Amino acid composition of napin and cruciferins.
| Napin, 2SSI_BRANA (P24565) | Cruciferin Cru 1 (P33525) | Cruciferin BnC2 (P33524) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number of amino acids in napin | Total % of amino acids | Number of amino acids in cruciferin Signal (1–23) | Number of amino acids in alpha chain (24–319) | Number of amino acid in beta chain (320–509) | Total amino acid count | Total % of amino acids | Number of amino acids in cruciferin signal (1–23) | Number of amino acids in alpha chain (24–306) | Number of amino acids in beta chain (307–496) | Total amino acids count | Total % of amino acids | |||
| Ala (A) | 6 | 5.5 | 2 | 11 | 16 | 29 | 6.933 | 2 | 16 | 15 | 33 | 7.43 | ||
| Arg (R) | 6 | 5.5 | 0 | 17 | 12 | 29 | 4 | 1 | 13 | 14 | 28 | 5.43 | ||
| Asn (N) | 4 | 3.6 | 1 | 15 | 16 | 32 | 5.9333 | 0 | 17 | 12 | 29 | 4.1 | ||
| Asp (D) | 1 | 0.9 | 0 | 13 | 5 | 18 | 2.3333 | 0 | 9 | 11 | 20 | 3 | ||
| Cys (C) | 8 | 7.3 | 1 | 4 | 2 | 7 | 2.266 | 0 | 3 | 2 | 5 | 0.73 | ||
| Gln (Q) | 21 | 19.1 | 0 | 63 | 16 | 79 | 9.9 | 0 | 49 | 12 | 61 | 7.86 | ||
| Glu (E) | 6 | 5.5 | 0 | 9 | 12 | 21 | 3.1 | 0 | 11 | 8 | 19 | 2.7 | ||
| Gly (G) | 6 | 5.5 | 2 | 35 | 11 | 48 | 8.766 | 1 | 39 | 13 | 53 | 8.3 | ||
| His (H) | 2 | 1.8 | 1 | 6 | 2 | 9 | 2.466 | 1 | 8 | 1 | 10 | 2.53 | ||
| Ile (I) | 6 | 5.5 | 0 | 15 | 14 | 29 | 4.1667 | 2 | 10 | 12 | 24 | 6.16 | ||
| Leu (L) | 6 | 5.5 | 6 | 18 | 18 | 42 | 13.9 | 5 | 18 | 18 | 41 | 12.53 | ||
| Lys (K) | 5 | 4.5 | 1 | 5 | 5 | 11 | 2.866 | 0 | 8 | 7 | 15 | 2.16 | ||
| Met (M) | 1 | 0.9 | 1 | 5 | 4 | 10 | 2.7 | 1 | 3 | 2 | 6 | 2.16 | ||
| Phe (F) | 6 | 5.5 | 1 | 7 | 5 | 13 | 3.1 | 2 | 13 | 5 | 20 | 5.3 | ||
| Pro (P) | 10 | 9.1 | 1 | 21 | 7 | 29 | 5.0333 | 0 | 17 | 8 | 25 | 3.4 | ||
| Ser (S) | 4 | 3.6 | 0 | 16 | 9 | 25 | 3.3667 | 4 | 17 | 17 | 38 | 10.76 | ||
| Thr (T) | 3 | 2.7 | 1 | 7 | 14 | 22 | 4.7 | 2 | 6 | 11 | 19 | 5.53 | ||
| Trp (W) | 2 | 1.8 | 0 | 4 | 1 | 5 | 0.633 | 0 | 3 | 2 | 5 | 0.73 | ||
| Tyr (Y) | 2 | 1.8 | 0 | 6 | 6 | 12 | 1.733 | 1 | 4 | 6 | 11 | 2.96 | ||
| Val (V) | 5 | 4.5 | 5 | 19 | 15 | 39 | 12 | 1 | 19 | 14 | 34 | 6.13 | ||
| Pyl (O) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Sec (U) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Number of amino acids | 110 | 23 | 296 | 190 | 99.9 | 23 | 283 | 190 | 99.96 | |||||
| Molecular weight | 12,690.53 | 2,408.04 | 32,938.47 | 21,192.11 | 2,541.05 | 3,0825.06 | 2,0960.66 | |||||||
| Theoretical pI | 8.71 | 8 | 7.17 | 6.94 | 8.52 | 7.95 | 8.6 | |||||||
| Total number of negatively charged sesidues (Asp + Glu) | 7 | 0 | 22 | 17 | 0 | 20 | 19 | |||||||
| Total number of Positively charged sesidues (Arg + Lys) | 77 | 1 | 22 | 17 | 1 | 21 | 21 | |||||||
| Atomic composition | ||||||||||||||
| C | 557 | 113 | 1,415 | 929 | 119 | 1,337 | 917 | |||||||
| H | 864 | 191 | 2,222 | 1,500 | 190 | 2,073 | 1,475 | |||||||
| N | 164 | 27 | 446 | 268 | 28 | 415 | 267 | |||||||
| O | 159 | 26 | 448 | 286 | 31 | 417 | 287 | |||||||
| S | 9 | 2 | 9 | 6 | 1 | 6 | 4 | |||||||
| Formula | C557H864N164O159S9 | C113H191N27O26S2 | C1415H2222N268O448S9 | C929H1500N268O286S6 | C119H190N28O31S1 | C1337H2073N415O417S6 | C917H1475N267O287S6 | |||||||
| Total Number of atoms | 1,753 | 359 | 4,540 | 2,989 | 369 | 4,248 | 2,950 | |||||||
| Aliphatic index | 61.18 | 173.48 | 65.81 | 97 | 140 | 63.71 | 90.84 | |||||||
| GRAVY | −0.661 | 1.778 | −0.903 | −0.226 | 1.274 | −0.753 | −0.326 | |||||||
GRAVY, Grand average of hydrophobicity.